Developing a tetO/TetR system in Neurospora crassa

Nitrate transport system in Neurospora crassa.

Nitrate uptake in Neurospora crassa has been investigated under various conditions of nitrogen nutrition by measuring the rate of disappearance of nitrate from the medium and by determining mycelial nitrate accumulation. The nitrate transport system is induced by either nitrate or nitrite, but is not present in mycelia grown on ammonia or Casamino Acids. The appearance of nitrate uptake activit...

Quelling in Neurospora crassa.

Heterocaryosis in Neurospora Crassa.

T IS possible by means of X-ray or ultraviolet treatment to produce mutant I strains of Neurospora crassa that are deficient in their abilities to synthesize specific vitamins, amino acids, or other essential substances (BEADLE and TATUM 1941; TATUM and BEADLE 1g4zb). It occurred to us that it might be possible to determine whether the mutant genes concerned in such haploid strains are recessiv...

Biotransformation of Hydrocortisone by Neurospora crassa

The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...

A derepressible active transport system for glucose in Neurospora crassa.

Two glucose uptake systems have been detected in ungerminated conidia of wild type Neurospora crassa, strain 74A; the high &ty system has been characterized. Uptake and substrate competition experiments showed that the high &inity system was relatively speciiic for D-glucose but also trsnsported the nonmetabolizable analogues 6-deoxy-D&ucase, 2-deoxy-D-glucose, and 3-O-methyl-D-glucose. The sys...